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Optimized enzyme mixture Nick translation utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix then incorporates labeled nucleotides as the polymerase examines or proofreads the nicked site.Individual templates produce consistent results in the standard 90-minutes reaction and result in an average probe length of 200 base pairs up to 500 base pairs.Assay Time 100 minutesSample Materials Supercoiled and linearized plasmid DNASupercoiled and linearized cosmid DNAPurified PCR products
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